Recent advances in enzyme-mediated peptide ligation

被引：1
作者：
Silin Xu [1 ]
Zhenguang Zhao [2 ]
Junfeng Zhao [1 ,3 ]
机构：
[1] Key Laboratory of Chemical Biology of Jiangxi Province,College of Chemistry and Chemical Engineering,Jiangxi Normal University
[2] The Institute of Chemistry,The Hebrew University of Jerusalem
[3] State Key Laboratory of Elemento-Organic Chemistry,Nankai University
来源：
Chinese Chemical Letters | 2018年 / 29卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Ligase; Peptide synthesis; Protein engineering; Enzyme-mediated peptide ligation; Site-specific protein modification;
D O I：
暂无
中图分类号：
O629.7 [α-氨基酸、肽类、蛋白质、核酸];
学科分类号：
070303 ; 081704 ;
摘要：
Artificial synthesis and site-specific modification of peptides and proteins has evolved into an indispensable tool for protein engineers and chemical biologists. Chemical and enzymatic approaches to peptide ligation are important alternatives of recombinant DNA technology for protein synthesis and modification. Although as old as that of chemical procedures, enzyme-mediated peptide ligation is far less developed than that of chemical counterpart due to the difficult availability of peptide ligase.Fortunately, this situation has been changed slowly with the fast development of biological techniques. In the past decades, several natural peptide ligases have been discovered. Protein engineering to improve the ligation efficiencies of the natural peptide ligase and to reverse the functionality of protease provide more powerful peptide ligases. In this review, the advances of enzyme-mediated peptide ligation and their application in protein synthesis and modification will be discussed.
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收藏
页码：1009 / 1016
页数：8
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