Comparison of Three ELISA Kits for the Differentiation of Foot-and-mouth Disease Virus-infected from Vaccinated Animals

被引:2
作者
Yi-mei CAO
机构
关键词
Foot-and-mouth disease virus (FMDV); Non-structural protein 3ABC; ELISA;
D O I
暂无
中图分类号
S852.5 [血清学];
学科分类号
090601 ;
摘要
A study was performed to validate 3 FMDV 3ABC-I-ELISA kits developed in China for the differentiation of FMDV infected and vaccinated animals. Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against nonstructural proteins (NSPs) of FMDV by commercial diagnosis kits, Ceditest FMDV-NS (Ceditest kit), UBI FMDV NON-STRUCTURAL PROTEIN ELISA DIRECTION INSERT (UBI kit) and a FMDV 3ABC-I-ELISA kit developed at the Lanzhou Veterinary Research Institute. The test parameters (sensitivity and specificity) of the three kits were determined, and the result obtained from FMD 3ABC-I-ELISA kit was compared with that obtained from two foreign kits. The results indicated that the coincidence rate between the FMDV 3ABC-I-ELISA and Ceditest kits was 98.05%, and the coincidence rate between the FMDV 3ABC-I-ELISA and UBI kits was 94.4%; the sensitivity of both Ceditest and FMDV 3ABC-I-ELISA kit was 100%. However, the sensitivity of the UBI kit was only 81.8%. With sera from naive or vaccinated non-infected animals, the specificity of all tests exceeded 90%.
引用
收藏
页码:74 / 79
页数:6
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  • [1] Improvement of a serodiagnostic strategy for foot-and-mouth disease virus surveillance in cattle under systematic vaccination: a combined system of an indirect ELISA-3ABC with an enzyme-linked immunoelectrotransfer blot assay[J] . I. E. Bergmann,V. Malirat,E. Neitzert,E. Beck,N. Panizzutti,C. Sánchez,A. Falczuk.Archives of Virology . 2000 (3)
  • [2] Differentiation of infection from vaccination in foot-and-mouth disease by the detection of antibodies to the non-structural proteins 3D, 3AB and 3ABC in ELISA using antigens expressed in baculovirus[J] . K. J. S?rensen,K. G. Madsen,E. S. Madsen,J. S. Salt,J. Nqindi,D. K. J. Mackay.Archives of Virology . 1998 (8)
  • [3] The non-structural polyprotein 3ABC of foot-and-mouth disease virus as a diagnostic antigen in ELISA to differentiate infected from vaccinated cattle
    DeDiego, M
    Brocchi, E
    Mackay, D
    DeSimone, F
    [J]. ARCHIVES OF VIROLOGY, 1997, 142 (10) : 2021 - 2033