Gene expression profiles of hepatoma cell line HLE

AIM:To investigate the global gene expression of cancerrelated genes in hepatoma cell line HLE using Atlas HumanCancer Array membranes with 588 well-characterized humangenes related with cancer and tumor biology.METHODS:Hybridization of cDNA blotting membrane wasperformed with32P-labeled cDNA probes synthesized fromRNA isolated from Human hepatoma cell line HLE and non-cirrhotic normal liver which was liver transplantation donor.Atlaslmage,a software specific to array,was used toanalyze the result.The expression pattern of some genesidentified by Atlas arrays hybridization was confirmed byreverse transcription polymerase chain reaction(RT-PCR)in 24 pairs of specimens and Northern blot of 4 pairs ofspecimens.RESULTS:The differential expression of cell cycle/growthregulator in hepatocellular carcinoma(HCC)showed astronger tendency toward cell proliferation with more than1.5-fold up-regulation of Cyclin C,ERK5,ERK6,E2F-3,TFDP-2 and CK4.The anti-apoptotic factors such as Akt-1 wereup-regulated,whereas the promotive genes of apoptosissuch as ABL2 were down-regulated.Among oncogene/tumors suppressors,SKY was down-regulated.Some genessuch as Integrin beta 8,Integrin beta 7,DNA-PK,CSPCP,byglycan,Tenacin and DNA Topo were up-regulated.Anumber of genes,including LAR,MEK1,eps15,TDGF1,ARHGDIA were down-regulated.In general,expression ofthe cancer progression genes was up-regulated,whileexpression of anti-cancer progression genes was down-regulated.These differentially expressed genes tested withRT-PCR were in consistent with cDNA array findings.CONCLUSION:Investigation of these genes in HCC ishelpful in disclosing molecular mechanism of pathogenesisand progression of HCC.For the first time few genes werediscovered in HCC.Further study is required for the preciserelationship between the altered genes and their correlationwith the pathogenesis of HCC.