Preliminary research on myosin light chain kinase in rabbit liver

被引：0

作者：

Bin Ren~1 Hua-Qing Zhu~2 Zhao-Feng Luo~1 Qing Zhou~2 Yuan Wang~2 Yu-Zhen Wang~1 1 Department of Biochemistry and Molecular Biology

机构：

来源：

World Journal of Gastroenterology | 2001年 / 06期

基金：

中国国家自然科学基金;

关键词：

myosin light chain kinase; liver; rabbit; enzyme activity; reverse transcription-polymerase chain reaction;

D O I：

暂无

中图分类号：

R575 [肝及胆疾病];

学科分类号：

1002 ; 100201 ;

摘要：

AIM:To study preliminarily the properties of myosin lightchain kinase(MLCK)in rabbit liver.METHODS:The expression of MLCK was detected byreverse transcdption-polymerase chain reaction(RT-PCR);the MLCK was obtained from rabbit liver,and its activitywas analyzed by γ-32p incorporation technique to detect thephosphorylation of myosin light chain.RESULTS:MLCK was expressed in rabbit liver,and theactivity of the enzyme was similar to rabbit smooth muscleMLCK,and calmodulin-dependent.When the concentrationwas 0.65 mg·L-1,the activity was at the highest level.CONCLUSION:MLCK expressed in rabbit liver may catalyzethe phosphorylation of myosin light chain,which may playimportant rolos in the regulation of hepatic cell functions.

引用

页码：868 / 871

页数：4