In situ formation of fluorescent silicon-containing polymer dots for alkaline phosphatase activity detection and immunoassay

被引：0

作者：

Guoyong Liu ^{[1
,2
]}

Jiahui Zhao ^{[1
,3
]}

Mengxia Yan ^{[1
,2
]}

Shuyun Zhu ^{[1
,4
]}

Wenchao Dou ^{[5
]}

Jian Sun ^{[1
]}

Xiurong Yang ^{[1
,2
]}

机构：

[1] State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences

[2] University of Science and Technology of China

[3] University of Chinese Academy of Sciences

[4] College of Chemistry and Chemical Engineering, Qufu Normal University

[5] Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University

来源：

Science China(Chemistry) | 2020年 / 04期

关键词：

fluorescent; polymer dots; ELISA; alkaline phosphatase; cardiac troponin Ⅰ;

D O I：

暂无

中图分类号：

O657.3 [光化学分析法（光谱分析法）]; TQ317 [高分子化合物产品]; R446.6 [免疫学检验];

学科分类号：

070302 ; 081704 ; 0805 ; 080502 ; 100208 ;

摘要：

The discovery and application of analyte-triggered fluorophore generation or fluorogenic reaction are significant and beneficial to the development of novel fluorescence(FL) analysis method. In this study, for the first time, we have reported a fluorogenic reaction to prepare fluorescent silicon-containing polymer dots(Si-PDs) by simply mixing N-[3-(trimethoxysilyl)propyl]ethylenediamine(DAMO) and hydroquinone(HQ) in aqueous solution at ambient temperature. Inspired by the alkaline phosphatase(ALP)-catalyzed hydrolysis of the substrate sodium 4-hydroxyphenyl phosphate(4-HPP) into HQ and the resultant HQcontrolled intense green Si-PDs generation, we have established a straightforward ALP activity assay by innovatively employing commercially available 4-HPP as the substrate. More significantly, the specific preparation method, clear formation mechanism and excellent performance enable the Si-PDs as well as its generation process to develop facile and attractive FL immunoassay.With the help of the universal ALP-based enzyme-linked immunosorbent assay(ELISA) platform and corresponding antibody, a convenient and conceptual ALP-based fluorescent ELISA has been constructed and applied in sensing cardiac troponin Ⅰ(cTnI),a well-known biomarker of acute myocardial infarction. Our research via in situ formation of fluorescent nanomaterials has great potential application in ALP activity assay, inhibitor screening, and disease diagnosis.

引用

页码：554 / 560

页数：7