Preparation and characterization of monoclonal antibodies against adenylate kinase

被引：0

作者：

王锡德

周筠梅

郭振泉

机构：

[1] State Key Laboratory of Biomacromolecules

[2] Beijing 100101

[3] Chinese Academy of Sciences

[4] China

[5] Beijing 100871

[6] Department of Biology

[7] Peking University

[8] Institute of Biophysics

来源：

Science in China(Series C:Life Sciences) | 1997年 / 06期

关键词：

adenylate kinase; monoclonal antibody; mixed antigen; solid-phase affinity; affinity in solution;

D O I：

暂无

中图分类号：

Q45 [内分泌生理学];

学科分类号：

071003 ;

摘要：

Six hybridoma cell lines that can continuously secrete monoclonal antibodies against adenylate kinase (AK) have been produced. The characteristics including the subclass and molecular weight of monoclonal antibodies manufactured by these strains are also determined. Further studies show that the two monoclonal antibodies McAb3D3 and McAMD8 bind easily with AK absorbed on microtitration plates, with affinity constants of 8.4 × 108 M-1 and 9.6 × 108 M-1, while their interactions to AK in solution are much weaker, with affinity constants of 7.0 × 104 M-1 and 3.9×106M-1, respectively. Thus, McAb3D3 and McAMD8 react preferentially to the immobilized AKs. Since pro-teins are often partially denatured when absorbed on microtitration plates, it is suggested that both McAb3D3 and McAMD8 are directed against non-native AK.

引用

页码：561 / 567

页数：7