Antidiabetic and antioxidant activity of ethyl acetate extract fraction of Moringa oleifera leaves in streptozotocin-induced diabetes rats via inhibition of inflammatory mediators

Objective:To evaluate the antioxidant and antidiabetic mechanism(s)of ethyl acetate extract fraction of Moringa oleifera(M.oleifera)leaves on streptozotocin-induced diabetes in male Sprague-Dawley rats.Methods:A total of 24 adult male rats were segregated randomly into four groups(6 rats each group).Streptozotocin-induced diabetes rats were given(oral gavage)ethyl acetate extract fraction of M.oleifera(200 mg/kg b.w.)for 30 d.The rats of control and experimental groups were sacrificed after 24 hours of final dose of treatment,to extract blood and pancreatic tissue for biochemical and histopathological analysis.Results:The ethyl acetate extract fraction of M.oleifera significantly reversed(P<0.05)the manifestation of streptozotocin on the levels of serum glucose&insulin,lipid profile,hepatic damage markers(alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase and lactate dehydrogenase),malondialdehyde formation,antioxidants(glutathione,Vitamin C&Vitamin E),antioxidant enzymes(superoxide dismutase,glutathione S-transferase,glutathione peroxidase and catalase)and pro-inflammatory cytokines(IL-1毬,TNF-毩&IL-6).Histopathological analysis of pancreatic tissues was in concurrence with the biochemical results.Conclusions:These findings support that M.oleifera leaves have potent therapeutic effect on diabetes mellitus via increasing antioxidant levels and inhibition of pro-inflammatory mediators.