A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

被引：0
作者：
ZHANG Zhen 1
2 Department of Isotope
机构：
来源：
Science Bulletin | 2011年 / 15期
基金：
中国国家自然科学基金;
关键词：
dual-label time-resolved fluoroimmunoassay; chloramphenicol; ractopamine; residue; swine tissue;
D O I：
暂无
中图分类号：
S859.84 [动物性食品中化学物残留];
学科分类号：
0906 ;
摘要：
A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples,using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu 3+) and samarium (Sm 3+),respectively.The detection limits for CAP and RAC were 0.06 and 0.25ng/g.The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5ng/g,and 69.8%-85.8% for RAC at spiking levels of 1-10ng/g.The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS,with correlation coefficients (R) between 0.92-0.98.
引用
收藏
页码：1543 / 1547
页数：5
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