Investigation of transient two-photon excited fluorescence in biological tissue

被引:0
作者
邱怡申 [1 ]
陈恩果 [2 ]
李高明 [1 ]
李晖 [1 ]
机构
[1] Key Laboratory of OptoElectronic Science and Technology for Medicine Ministry of Education,Fujian Provincial Key Laboratory for Photonics Technology,Fujian Normal University
[2] Department of Optical Engineering,Zhejiang University
关键词
time; ab;
D O I
暂无
中图分类号
TN24 [激光技术、微波激射技术];
学科分类号
0803 ; 080401 ; 080901 ;
摘要
The fluorescence power from biological tissue excited by a femtosecond laser pulse compared with excitation power does not appear to obey a simple quadratic relationship given by the steady non-linear theory.A more reliable analysis is developed based on transient two-photon absorption because the response time of two-photon absorption is longer than the width of a femtosecond pulse.Good agreement is obtained between the theoretical analysis and the experimental results of fluorescence power versus excitation power.This letter offers potential value to non-linear optics in biological tissues.
引用
收藏
页码:48 / 50
页数:3
相关论文
共 13 条
  • [1] Shen Y R.The Principles of Nonlinear Optics. . 1984
  • [2] Denk W,Strickler J,Webb WW.Two-photon laser scanning fluorescence microscopy. Science . 1990
  • [3] H. Quan,Z. Guo. Optics Express . 2004
  • [4] V. E. Centonze,J. G. White. Biophysical Journal . 1998
  • [5] Z. Song,T. Nakajima. Chinese Optics Letters . 2011
  • [6] Peter T.C So,Chen Y.Dong,Bstty R.Masters,Keith M.Berland.Two-Photon Excitation Fluorescence Microsopy. Annual Review of Biomedical Engineering . 2000
  • [7] F. Helmchen,W. Denk. Nat. Methoods . 2005
  • [8] Zipfel WR,Williams RM,Webb WW.Nonlinear magic: multiphoton microscopy in the biosciences. Nature Biotechnology . 2003
  • [9] Xu C,Zipfel W,Shear J B,et al. Proceedings of the National Academy of Sciences of the United States of America . 1996
  • [10] R Cicchi,FS Pavone.Non-linear fluorescence lifetime imaging of biological tissues. Analytical and Bioanalytical Chemistry . 2011