SLAM family predicting the initiation potential of human acute lymphoblastic leukemia in NOD/SCID mice

被引：0

作者：

WANG Na ^{[1
]}

XIAO Fei ^{[2
]}

LIU Jinping ^{[3
]}

WANG Di ^{[1
]}

GENG Zhe ^{[1
]}

WANG Jin ^{[1
]}

MA Shuyan ^{[3
]}

SHU Lili ^{[3
]}

CHEN Taiping ^{[3
]}

ZHOU Jianfeng ^{[1
]}

HUANG Liang ^{[1
]}

机构：

[1] Department of Hematology Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei ,China

[2] Department of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei ,China

[3] Crown Bioscience Inc,Beijing ,China

来源：

中华医学杂志（英文版） | 2011年 / 19期

关键词：

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Background The SLAM family recently has been reported to show an important biological role in lymphocytedevelopment and immunological function,and it is efficient to highly purify hematopoietic stem cells using a simplecombination of SLAM family members.To elucidate the presence of this family on acute lymphoblastic leukemia（ALL）,as well as its relationship with the leukemia-initiating potential,we analyzed the expression pattern of this familymembers on human ALL progenitor cells,combined with serial xenotransplantation assay.Methods Expression analysis was carried out by flow cytometry.We combined the expression pattern of human CD150,CD244and CD48with serial xenotransplantation of B-ALL progenitor cells to indicate their relationship.Results CD48and CD244were expressed on most B-ALL progenitor cells,the percentage being（93.08±6.46）% and（63.37±29.31）%,respectively.Interestingly,the proportion of CD150+cells declined obviously in engrafted cases(（24.94±7.32）%)compared with non-engrafted cases(（77.54±5.93）%,P<0.01),which indicated that only blast cells withlow percentage of CD150+population were able to reconstitute leukemia into primary,secondary and tertiary NOD/SCIDmice.Conclusions SLAM family members are present on B-ALL progenitor cells and the leukemia-initiating potential ofleukemic blasts is correlated negatively with the proportion of CD150+cells,the percentage of which can serve as a usefulpredictor for engraftment success of B-ALL to immune deficient mice.

引用

页码：3074 / 3079

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