Evaluation on Inducible Effect of pOp6 Promoter in Transgenic Rice

被引：0

作者：

Rouyi CHEN ^{[1
,2
]}

Changxiang ZHENG ^{[1
]}

Jiang CHENG ^{[1
]}

Minna PAN ^{[1
]}

Mariena KETUDAT-CAIRNS ^{[2
]}

机构：

[1] Guizhou Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences

[2] School of Biotechnology, Suranaree University of Technology

来源：

Agricultural Science & Technology | 2014年 / 15卷 / 05期

关键词：

POp6; promoter; GUS activity; Inducible effect;

D O I：

10.16175/j.cnki.1009-4229.2014.05.024

中图分类号：

S511 [稻];

学科分类号：

0901 ;

摘要：

In order to analyze the Os1bglu4 phenotype,the inducible promoter of the transgenic rice which knock-down the Os1bglu4 expression was assessed.The result showed that 30μM dexamethasone(DEX)had the stronger induction effect than10μM DEX byβ-Glucuronidase(GUS)staining.qRT-PCR further verified the Os1bglu4 gene deletion.The effect of DEX and its solvent absolute ethanol on seed development was measured,and no significant effect was observed.The conclusion is that final concentration of DEX at 30μM is suitable for pOp6 promoter induction.

引用

页码：742 / 744

页数：3

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