In vitro and in vivo evaluation of the antiangiogenic activities of Trigonella foenum-graecum extracts

被引：0
作者：
Zina A.Habib-Martin [1 ]
Hana M.Hammad [1 ]
Fatma U.Afifi [2 ]
Malek Zihlif [3 ]
Hamzeh J.Al-Ameer [3 ]
Mohanad M.Saleh [3 ]
Ismail F.Abaza [2 ]
Zeyad D.Nassar [4 ,5 ]
机构：
[1] Department of Biology,School of Science,The University of Jordan
[2] Department of Pharmaceutical Sciences,School of Pharmacy,The University of Jordan
[3] Department of Pharmacology,School of Medicine,The University of Jordan
[4] School of Medicine and Freemasons Foundation Centre for Men's Health,University of Adelaide
[5] South Australian Health and Medical Research Institute
来源：
Asian Pacific Journal of Tropical Biomedicine | 2017年 / 7卷 / 08期
关键词：
Fenugreek; MCF7; Angiogenesis; Rat aortic ring assay; CAM assay; MTT assay;
D O I：
暂无
中图分类号：
R285.5 [中药实验药理];
学科分类号：
1008 ;
摘要：
Objective: To assess the antiangiogcnic activity of fenugreek.Methods: Different fractions of fenugreek crude extracts were prepared and their antiangiogenic properties were assessed using the ex vivo rat aortic ring assay and in vivo chicken embryo chorioallantoic membrane(CAM) assay. They were investigated for their direct cytotoxic activity in the MCF7 cells using the MTT assay.Results: The ethanol extract showed 100% inhibition of blood vessel outgrowth from primary tissue explants in the rat aortic ring assay at a concentration of 100μg/mL while the other extracts did not show significant antiangiogenic activity. The ethanol extract was therefore investigated at varying concentrations and exhibited a significant dose dependent effect. The CAM assay coincided with the results of the aortic ring assay as ethanol extract showed a significant inhibition of formation of new blood vessels. The extracts only showed anti-proliferative activity at the highest concentration of 400μg/mL towards MCF7 breast cancer cell lines in the MTT assay.Conclusions: Findings of the both assays confirmed that the ethanol extract inhibited vascularization significantly. Further studies on the ethanol extract would be beneficial in isolating the active ingredient responsible for the inhibition.
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页码：732 / 738
页数：7
相关论文
共 11 条
  • [1] Optimization of Cat’s Whiskers Tea （ Orthosiphon stamineus ） Using Supercritical Carbon Dioxide and Selective Chemotherapeutic Potential against Prostate Cancer Cells[J] . Fouad Saleih R. Al-Suede,Mohamed B. Khadeer Ahamed,Aman S. Abdul Majid,Hussin M. Baharetha,Loiy E. A. Hassan,Mohd Omar A. Kadir,Zeyad D. Nassar,Amin M. S. Abdul Majid,K. B. Harikumar.Evidence-Based Complementary and Alternative Medicine . 2014
  • [2] Angiogenic factors as potential drug target: Efficacy and limitations of anti-angiogenic therapy[J] . Rajesh N. Gacche,Rohan J. Meshram.BBA - Reviews on Cancer . 2014 (1)
  • [3] VEGF Signal System: The Application of Antiangiogenesis[J] . Xuewu Liang,Fuming Xu,Xiaoguang Li,Chunhua Ma,Yingjie Zhang,Wenfang Xu.Current Medicinal Chemistry . 2014 (7)
  • [4] Angiogenesis and Collagen Type IV Expression in Different Endothelial Cell Culture Systems
    Bahramsoltani, M.
    Slosarek, I.
    De Spiegelaere, W.
    Plendl, J.
    [J]. ANATOMIA HISTOLOGIA EMBRYOLOGIA, 2014, 43 (02) : 103 - 115
  • [5] Cat's Whiskers Tea (Orthosiphon Stamineus) Extract Inhibits Growth of Colon Tumor in Nude Mice and Angiogenesis in Endothelial Cells via Suppressing VEGFR Phosphorylation
    Ahamed, Mohamed B. Khadeer
    Aisha, Abdalrahim F. A.
    Nassar, Zeyad D.
    Siddiqui, Jamshed M.
    Ismail, Z.
    Omani, S. M. S.
    Parish, C. R.
    Majid, A. M. S. Abdul
    [J]. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2012, 64 (01): : 89 - 99
  • [6] Anti-inflammatory and antioxidative effects of mucilage of Trigonella foenum graecum （Fenugreek） on adjuvant induced arthritic rats[J] . G. Sindhu,M. Ratheesh,G.L. Shyni,Bala Nambisan,A. Helen.International Immunopharmacology . 2011 (1)
  • [7] Drug discovery from medicinal plants
    Balunas, MJ
    Kinghorn, AD
    [J]. LIFE SCIENCES, 2005, 78 (05) : 431 - 441
  • [8] Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts
    Wang, SS
    Zheng, ZG
    Weng, YQ
    Yu, YJ
    Zhang, DF
    Fan, WH
    Dai, RH
    Hu, ZB
    [J]. LIFE SCIENCES, 2004, 74 (20) : 2467 - 2478
  • [9] Drug inhibition of angiogenesis
    Madhusudan, S
    Harris, AL
    [J]. CURRENT OPINION IN PHARMACOLOGY, 2002, 2 (04) : 403 - 414
  • [10] Anti-inflammatory and antipyretic effects of Trigonella foenum-graecum leaves extract in the rat[J] . A. Ahmadiani,M. Javan,S. Semnanian,E. Barat,M. Kamalinejad.Journal of Ethnopharmacology . 2001 (2)
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