Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

被引：0
|
作者：
LI Bing1
2. Institute of Molecular Cell Biology
机构：
来源：
Chinese Science Bulletin | 2003年 / 03期
关键词：
calmodulin; heat shock transcription factor; DNA-binding activity; maize; wheat; tomato;
D O I：
暂无
中图分类号：
Q523 [脱氧核糖核酸（DNA）];
学科分类号：
摘要：
The DNA-binding activity of heat shocktranscription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific toCaM, to a whole cell extract reduced bind of the HSF to the heat shock element (HSE) with maize, and the re-addition of CaM to the sample restored the activity of the HSF forbinding to HSE. In addition, DNA-binding activity of the HSF was also induced by directly adding CaM to a whole cell extract at non-HS temperature with maize. Similar resultswere obtained with wheat and tomato. Our observationprovide the first example of the involvement of CaM inregulation of the DNA-binding activity of the HSF.
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页码：255 / 258
页数：4
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