Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

被引：0

作者：

LI Bing LIU Hongtao MU Ruiling SUN Daye ZHOU Rengang Institute of AgroPhysics Physiology and Biochemistry HebeiAcademy of Agricultural and Forestry Sciences Shijiazhuang China Institute of Molecular Cell Biology Hebei Normal University Shijiazhuang China ^{[1
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机构：

来源：

Chinese Science Bulletin | 2003年 / 03期

关键词：

calmodulin; heat shock transcription factor; DNA-binding activity; maize; wheat; tomato;

D O I：

暂无

中图分类号：

Q523 [脱氧核糖核酸（DNA）];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA-binding activity of heat shocktranscription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific toCaM, to a whole cell extract reduced bind of the HSF to the heat shock element (HSE) with maize, and the re-addition of CaM to the sample restored the activity of the HSF forbinding to HSE. In addition, DNA-binding activity of the HSF was also induced by directly adding CaM to a whole cell extract at non-HS temperature with maize. Similar resultswere obtained with wheat and tomato. Our observationprovide the first example of the involvement of CaM inregulation of the DNA-binding activity of the HSF.

引用

页码：255 / 258

页数：4

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