Bile anaerobic bacteria detection and antibiotic susceptibility in patients with gallstone

OBJECTIVE: To detect bile anaerobic bacteria and antibiotic susceptibility in 59 patients with gallstoneswho had had cholecystectomy.METHODS: BACT/ALERT 120 microbe detection system and SCEPTOR microbe detection system wereused to detect bile anaerobic bacteria, antibiotic susceptibility.RESULTS: The ratio of anaerobic bacteria to the patients examined was 52.5% (31/59). Obligateanaerobe bile culture showed positive results in 4 patients. B. fragilis (37.8%) was the major type ofanaerobic bacteria in bile. Most (81.8%) of anaerobic bacteria were sensitive to metronidazole, andimipenem was suitable for β-lactamase bacteria.CONCLUSIONS: Culture of anaerobic bacteria in logarithmic phase can improve the positive rate of theculture. There are some relations between anaerobic infection and gallstone formation.