Effect of curcumin on proliferation and cycle of human ECa-109 cells

OBJECTIVE To investigate the mechanism of curcumin on Inhibition of cell proliferation and apoptosis induction in esophageal cancer cells.METHODS Eca-109 cell growth inhibiting rates induced by curcumin were investigated by using CCK-8 assay.Eca-109 cell growth inhibiting rates induced by curcumin were investigated by using DNA gel electrophoresis for detection of DNA Ladder,electron microscopy observation technology for detection of ultrastructure and automatic fluorescence microplate reader.RESULTS Curcumin can inhibit cell proliferation growth inhibition rate had increased in a dose-and time-dependent manner.Caspase 3 activity assay detected that OD value with 20 μmol·L-1,40 μmol·L-1 of curcumin for 24 h,compared with the control group,the difference was statistically significant(P<0.05).Electron microscopy showed that curcumin can damaging cellular ultrastructure.CONCLUSION Curcumin can damage cellular ultrastructure of Eca-109 cells,Inhibit cell proliferation and induce its apoptosis.