SYNTHESIS OF DELTA SLEEP-INDUCING PEPTIDE(DSIP) AND ITS PHYSIOLOGICAL ACTIVITY

Protected nonapeptide—Delta Sleep-Induclng Peptide（DSIP）(Boc·Trp·Ala·Gly·Gly·Asp·Ala·Ser·Gly·Glu-OBu) has been synthesized by classical method. The product has been treated with TFA and purified on DEAE-Sephadex-A25 coltunn, pure free nonapeptide obtained and a to β transposition of Asp-residue found to be absent. It has been assayed by electrophoresis at pH 3.8, microcrystallinecellulose TLC and HPLC. The physiological activities of synthetic DSIP are performed on rabbits by using intravenous administration or mesodiencephalic ventricular infusion. Its function Of intensifying δ and σ waves on rabbit’s electroencephalogram（EEG） is evident. There is no concomitant increase of δ- and σ-enhancing effect following mesodiencephalic ventricular infusion of 10 or 20 times higher than 5 μg/rabbit doses. Results of 6-day consecutive intravenous administration（50μg/kg） indicate that there is no obvious sign of adaptation to DSIP. Results suggest that the physiological function of endogenous sl