Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines

被引：0

作者：

Hataipan Phienwej ^{[1
]}

Ih-si Swasdichira ^{[1
]}

Surattana Amnuoypol ^{[2
]}

Prasit Pavasant ^{[1
,3
]}

Piyamas Sumrejkanchanakij ^{[1
,3
]}

机构：

[1] Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University

[2] Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University

[3] Department of Anatomy, Faculty of Dentistry, Chulalongkorn University

来源：

Asian Pacific Journal of Tropical Biomedicine | 2015年 / 09期

关键词：

Tinospora crispa; Matrix metalloproteinase 13; Cell migration; Squamous cell carcinoma; Cell line; Phytochemical;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

Objective: To investigate the effect of Tinospora crispa(T. crispa) extract on matrix metalloproteinase 13(MMP-13) expression and cell migration. Methods: The cytotoxicity of T. crispa extract was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on head and neck squamous cell carcinoma(HNSCC) cell lines. The effect on expression of MMP-13 was analysed by RT-PCR and ELISA. The migration was assessed by wound healing assay. Results: MMP-13 m RNA was highly expressed in the metastatic human HNSCC cell lines, HN22 and HSC-3. T. crispa extract at a concentration of 100.0 μg/m L caused about 50% reduction of cell survival. T. crispa extract at a non-toxic concentration of 12.5, 25.0 and 50.0 μg/m L signii cantly suppressed MMP-13 m RNA expression and secreted MMP-13 in both HN22 and HSC-3. The expression of tissue inhibitors of metalloprotease by HSC-3 cells was attenuated by 25.0 and 50.0 μg/m L of T. crispa extract. Addition of the extract to cells in a wound healing assay showed inhibition of cell migration by HN22 cells. Conclusions: These data suggest that T. crispa could be considered as a potential therapeutic drug to prevent metastasis of HNSCC.

引用

页码：713 / 717

页数：5

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