共 1 条
[1]
Guarente,L.UASs and enhancers; commn mechanism of transcriptional activation in yeast and mammals. Cell . 1988
Transcription reactions of yeast RNA polymerase II in vitro
被引：0
作者：
赵宇
敖世洲
机构：
[1] StateKeyLaboratoryofMolecularBiology,ShanghaiInstituteofBiochemistry,ChineseAcademyofSciences,Shanghai,China,StateKeyLaboratoryofMolecularBiology,ShanghaiInstituteofBiochemistry,ChineseAcademyofSciences,Shanghai,China
关键词：
transcription in vitro;
ADHl and PHO5 promoters;
αα-amanitin;
RNA probes;
D O I：
暂无
中图分类号：
Q55 [酶];
学科分类号：
071010 ;
081704 ;
摘要：
<正> The transcription reactions in vitro of yeast ADHl and PHO5 gene promoters are investigated by means of a yeast crude nuclear extract. Using specific RNA probes, the transcription products of these 2 promoters have been first obtained. A low concentration of α-amanitin is highly inhibitory. The transcription of the PHO5 gene was initiated in vitro at or near the sites used in vim. The transcription products increase with the amount of the template and reach the maximum at certain concentrations of the template. The deletion of the yeast promoter sequences abolishes the reaction.
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页码：697 / 704
页数：8
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