Effect of WeiJia on carbon tetrachloride induced chronic liver injury

被引：0

作者：

Pik-Yuen Cheung ^{[1
]}

QiZhang ^{[1
]}

Gan-Rong Bai ^{[1
]}

Chi-Chun Fong ^{[1
]}

Lin Shi ^{[1
]}

Yue-Feng Shi ^{[1
]}

Meng-SuYang ^{[1
]}

DepartmentofBiology ^{[1
]}

Ya-Ou Zhang ^{[2
]}

Marie Chia-Mi Lin ^{[3
]}

Bernard Chan ^{[4
]}

Jay Chun ^{[4
]}

Hsiang-Fu Kung ^{[5
]}

机构：

[1] Department of Biology and Chemistry,City University of Hong Kong 83 Tat Chee Avenu,Kowloon,Hong Kong SAR,China

[2] Life Science Division,Tsinghua University Graduate School at Shenzhen,Tsinghua Campus,University Town,Shenzhen,China

[3] Institute of Molecular Biology,The University of Hong Kong,Pokfulam,Hong Kong,SAR,China

[4] Life Tec Enterprise Limited,Central,Hong Kong,SAR,China

[5] The Centre for Emerging Infectious Diseases,Faculty of Medicine,The Chinese University of Hong Kong,Shatin,N.T.,Hong Kong SAR,China

来源：

World Journal of Gastroenterology | 2006年 / 12期

关键词：

WeiJia; Carbon tetrachloride; Liver fibrosis;

D O I：

暂无

中图分类号：

R575 [肝及胆疾病];

学科分类号：

1002 ; 100201 ;

摘要：

AIM:To study the effect of WeiJia on chronic liver injuryusing carbon tetrachloride(CCl4)induced liver injuryanimal model.METHODS:Wistar rats weighing 180-220g were ran-domly divided into three groups:normal control group(Group A),CCl4induced liver injury control group(GroupB)and CCl4induction with WeiJia treatment group(GroupC).Each group consisted of 14 rats.Liver damage andfibrosis was induced by subcutaneous injection with 40%CCl4in olive oil at 3 mL/kg body weight twice a week foreight weeks for Groups B and C rats whereas olive oilwas used for Group A rats.Starting from the third week,Group C rats also received daily intraperitoneal injectionof WeiJia at a dose of 1.25 μg/kg body weight.Animalswere sacrificed at the fifth week(4 male,3 female),andeighth week(4 male,3 female)respectively.Degree offibrosis were measured and serological markers for liverfibrosis and function including hyaluronic acid(HA),typeIV collagen(CIV),γ-glutamyl transferase(γ-GT),alanineaminotransferase(ALT)and aspartate aminotransferase(AST)were determined.Alpha smooth muscle actin (α-SMA)and proliferating cell nuclear antigen(PCNA)immunohistochemistry were also performed.RESULTS:CCl4induction led to the damage of liver anddevelopment of fibrosis in Group B and Group C ratswhen compared to Group A rats.The treatment of WeiJiain Group C rats could reduce the fibrosis condition sig-nificantly compared to Group B rats.The effect could beobserved after three weeks of treatment and was moreobvious after eight weeks of treatment.Serum HA,CIV,ALT,AST and γ-GT levels after eight weeks of treatmentfor Group C rats were 58±22 μg/L(P<0.01),57±21 μg/L(P<0.01),47±10 U/L(P<0.01),139±13 U/L(P<0.05)and 52±21 U/L(P>0.05)respectively,similar to normalcontrol group(Group A),but significantly different fromCCl4induced liver injury control group(Group B).An in-crease in PCNA and decrease in α-SMA expression levelwas also observed.CONCLUSION:WeiJia could improve liver function andreduce liver fibrosis which might be through the inhibi-tion of stellate cell activity.

引用

页码：1912 / 1917

页数：6

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