Correlation Analysis between Taurine Content of Brain Tissue Determined by Amino Acid Analyzer and the Age of Growth in Mice

Objective:To observe the correlation between taurine(Tau)content in mouse brain tissue and the age of growth,and to provide normal reference data for the change of Tau content of brain under different disease state. Methods:We selected SPF level and Kunming species mice,the age range of which was from 1 week to 34 weeks(1 w,2 w,3 w,4 w,5 w,6 w,7 w,8 w,9 w,10 w,15 w,18 w,22 w,26 w,31 w,and 34 w),using the amino acid analyzer A300,continuously measured the content of Tau in the mice brain,and observed the correlation between the level of Tau in the brain tissue of male and female mice and the age of growth. Then we compared the differences of Tau content of male and female mice,and also compared the difference of Tau content in cerebral cortex and hippocampus. Results:The change trend of Tau content in the brain of mice was as follows:Tau content was declining continuously in 1～4 weeks(0.266→0.127 mg),fluctuating in a certain range(0.119→0.158 mg) in 4～26 weeks,and stable in 26～34 weeks(0.110→0.125 mg). Among the 16 time points in this study,compared with the female mouse cortex,the Tau content in the cortex of male mice was lower at 31 weeks. While compared with the hippocampus of female mice,the Tau content in the hippocampus of male mice was lower at 2 weeks and higher at 15 weeks(P<0.05). And compared with male mice hippocampus,the content of Tau in the cortex of male mice was higher at 2 and 22 weeks,and lower at 3,4,6,and 15 weeks. Also compared with the hippocampus of female mice,the content of Tau in the cortex of female mice was higher at 26 weeks,and lower at 2 to 4 and 18 weeks(P<0.05). Conclusions:The Tau content first decreased,then fluctuated,and reached a trough at 4～6 weeks,and remained a low value from 26 weeks. There was no significant difference between Tau content of male and female mice. But compared with hippocampus,the cortical tissue showed significant differences of Tau content before 6 weeks. The reasons and mechanisms for the change of Tau content need to be further studied.