Investigation of immunogenicity of cryopreserved limbal stem cells

被引：0

作者：

Yan Zhang 1

机构：

来源：

International Journal of Ophthalmology | 2011年 / 06期

关键词：

rabbit limbal stem cells; cryopreservation; immunogenicity; explant;

D O I：

暂无

中图分类号：

R329 [人体组织学];

学科分类号：

100101 ;

摘要：

· AIM: To investigate changes in immunogenicity of cryopreserved limbal stem cells. · METHODS: Cryopreserved limbal stem cells, fresh primary limbal stem cells and blank controls were inoculated subcutaneously in C57BL-6 mice and the percentage of CD25 cells in limbal explants was determined by flow cytometry at day 21 post inoculation. Morphological studies were performed by light and electron microscopy of limbal explant sections. · RESULTS: The number of regional and systemic lymphocytes derived from cryopreserved limbal stem cells was lower than that from fresh primary limbal stem cells. · CONCLUSION: Lymphocytes derived from cryopreserved limbal stem cells showed changes in immunogenicity, but the significance is unknown. The cryopreservation and thawing methods await further study. ·

引用

页码：590 / 593

页数：4

共 5 条

[1] 低温冷冻对角膜缘干细胞生物学特性的影响 [J].

李翠霞 ;

孙荔 ;

洪晶 .

眼科, 2010, 19 (03) :189-193

[2] 超低温(-196℃)保存角膜缘上皮细胞的生物活性研究 [J].

高明宏 ;

张劲松 ;

蓝平 .

中国实用眼科杂志, 2006, (06) :658-662

[3] 深低温保存角膜缘干细胞活性实验研究 [J].

周善璧 ;

陈家祺 ;

黄挺 ;

陈龙山 ;

许丽英 .

重庆医学, 2000, (06) :481-482

[4] Cryopreservation of human limbal stem cells ex vivo expanded on amniotic membrane [J].

Yeh, Hui-Jung ;

Yao, Chao-Ling ;

Chen, Hsin-I ;

Cheng, Huey-Chuan ;

Hwang, Shiaw-Min .

CORNEA, 2008, 27 (03) :327-333

[5]

Corneal epithelial stem cells: characterization, culture and transplantation[J] . Sajjad Ahmad,Francisco Figueiredo,Majlinda Lako.Regenerative Med. . 2006 (1)

← 1 →