Construction,expression and characterization of tissue-type plasminogen activator mutants

被引：0

作者：

刘士辉 ^{[1
]}

黄培堂 ^{[1
]}

黄翠芬 ^{[1
]}

机构：

[1] Beijing Institute of Biotechnology,Beijing 100850,China

来源：

Science in China(Series B) | 1995年 / 38卷 / 11期

关键词：

tissue-type plasminogen activator; mutant; plasma haft-life; PAI-1;

D O I：

暂无

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

Three tissue-type plasminogen activator(t-PA)mutants were constructed by recombinant andsite-directed mutagenesis techniques.They are del(296—302)with deletion of PAI-1 binding site,N117Q/N184Qwith deglycosylation of K1 and K2 domains,and their combination mutant designated as GGI.Then these threemutants were suocessfully transiently expressed in COS-7 ceils,and GGI was further stably expressed in CHOcells.The biological characterization of the expression products indicated that del(296—302)and GGIpossessed the resistance to inhibition by PAI-1.In addition,the specific activity of GGI was increased byabout 46,the plasma half-life was prolonged by about one fold,while its affinity for fibrin was not affected.

引用

页码：1341 / 1348

页数：8