Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine

被引：0

作者：

Wei JINLefeng QUQin CHENXinzhong CHANGJiong WUZhimin SHAO Breast Cancer InstituteCancer HospitalDepartment of OncologyShanghai Medical CollegeInstitute of Biomedical ScienceFudanUniversityShanghai China ^{[2
,3
,4
,2
,2
,2
,5
,2
,200032
]}

Department of Vascular Surgery and Institute of Vascular Surgery of PLAChanghai HospitalShanghai China ^{[3
,200433
]}

Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai China ^{[4
,201203
]}

机构：

来源：

Acta Pharmacologica Sinica | 2007年 / 06期

关键词：

QGY-7703; cells; homoharringtonine; apoptosis; gene expression;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

<正> Aim:To classify the genes responsible for apoptosis in QGY-7703 cells in-duced by homoharringtonine (HHT).Methods:Apoptosis in QGY-7703 cellsinduced by HHT was demonstrated by DNA fragmentation and morphologicalobservation,cDNA microarray technology was used to detect gene transcription,and the result of microarrays for genes was confirmed by RT-PCR.Results:Seventy-eight individual mRNA were identified and their transcription levelschanged significantly.Those genes,of which 68% were upregulated and 32%were downregulated,were partially related to apoptosis.They were mostlyoncogenes,tumor suppressors,enzymes,and kinases.Conclusion:HHT is apotential drug in the treatment of liver cancer.TGF-β,TNF,FAS,p38MAPK,and p53 apoptosis signaling pathways were activated during apoptosis in QGY-7703 cells.Such inducible genes may play important roles in apoptosis anddeserve to be further studied.

引用

页码：859 / 868

页数：10

共 7 条

[1] The modulation of radiation-induced cell death by genistein in K562 cells:Activation of thymidine kinase 1 [J].

Min Ho JEONG ;

Young Hee JIN ;

Eun Young KANG ;

Wol Soon JO ;

Hwan Tae PARK ;

Jae Dong LEE ;

Yeo Jin YOO ;

Soo Jin JEONG .

CellResearch, 2004, (04) :295-302

[2] Overexpression of the chromosome 21 transcription factor Ets2 induces neuronal apoptosis [J].

Wolvetang, EJ ;

Bradfield, OM ;

Hatzistavrou, T ;

Crack, PJ ;

Busciglio, J ;

Kola, I ;

Hertzog, PJ .

NEUROBIOLOGY OF DISEASE, 2003, 14 (03) :349-356

[3] A novel zinc finger-containing RNA-binding protein conserved from fruitflies to humans [J].

Jackson, FB ;

Banfi, S ;

Guffanti, A ;

Rossi, E .

GENOMICS, 1997, 41 (03) :444-452

[4]

Id3 induces growth arrest and caspase-2-dependent apoptosis in B lymphocyte progenitors. Kee BL. J Immunol . 2005

[5]

Chromosomal localization of the genes encoding the p50/p105 subunits of NF-κB （NFKB2） and the IκB/MAD-3 （NFKBI） inhibitor of NF-κB to 4q24 and 14q13, respectively. Le,Beau,MM,Ito,C,Cogswell,P,III,Espinosa,R,Fernald,AA,Jr,Baldwin,AS. Genomics . 1992

[6]

Molecular cloning and characterization of NF-IL3A,a transcriptional activator of the human interleukin-3 promoter. Zhang W,Zhang J,Kornuc M,Kwan K,Frank R,Nimer SD. Molecular and Cellular Biology . 1995

[7]

Homoharringtonine mediates myeloid cell apoptosis via upregulation of proapoptotic bax and inducing caspase-3-mediated cleavage of poly （ADP-ribose） polymerase (PARP. Yinjun L,Jie J,Weilai X, et al. American Journal of Hematology . 2004

← 1 →