Production and Characterization of Monoclonal Antibodies of Shrimp White Spot Syndrome Virus Envelope Protein VP28

被引：0

作者：

Wangang GU Junfa YUAN Gelin XU Lijuan LI Ni LIU Cong ZHANG Jianhong ZHANG Zhengli SHI State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan China ^{[430071
]}

Wuhan Institute of Biological Products Ministry of Health Wuhan China ^{[430060
]}

College of Life Science Central China Normal University Wuhan China ^{[430079
]}

机构：

来源：

Virologica Sinica | 2007年 / 01期

关键词：

White spot syndrome virus (WSSV); Mab; Envelope; Localization; Neutralization;

D O I：

暂无

中图分类号：

S945.1 [虾类];

学科分类号：

0906 ;

摘要：

<正>BALB/c mice were immunized with purified White spot syndrome virus (WSSV). Six monoclonal antibody cell lines were selected by ELISA with VP28 protein expressed in E. coli. in vitro neutralization experiments showed that 4 of them could inhibit the virus infection in crayfish. Western-blot suggested that all these monoclonal antibodies were against the conformational structure of VP28. The monoclonal antibody 7B4 was labeled with colloidal gold particles and used to locate the VP28 on virus envelope by immunogold labeling. These monoclonal antibodies could be used to develop immun-ological diagnosis methods for WSSV infection.

引用

页码：21 / 25

页数：5

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