Isolation,purification and characterization of secondary structure of antifreeze protein from Ammopiptanthus mongo-licus

被引：3
|
作者：
FEI Yunbiao WEI Lingbo
Institute of Physical Chemistry
机构：
来源：
Chinese Science Bulletin | 2001年 / 06期
基金：
中国国家自然科学基金;
关键词：
Ammopiptanthus mongolicus; AFP; THA; DSC; CD;
D O I：
暂无
中图分类号：
Q945 [植物生理学];
学科分类号：
0903 ;
摘要：
Antifreeze protein (AFP) from Ammopiptanthus mongolicus leaves was isolated and purified with DEAE cellulose 52, Sephacryl 300, heat treatment and preparative isotachophoresis. The molecular weight of AFP was 50 ku by SDS-PAGE measurement. The average thermal hysteresis activity (THA) of AFP was 0.35℃ at 5 mg/mL using a differential scanning calorimeter (DSC). This AFP showed heated stability. From circular dichroism (CD) of AFP spectral data from 195-240 nm, a well-defined secondary structure of 11% a-helix, 34% antiparallel β-sheet and 55% random coil for the plant AFP was deduced.
引用
收藏
页码：495 / 498
页数：4
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