Protective effects of upregulated HO-1 gene against the apoptosis of human retinal pigment epithelial cells in vitro

被引：0
作者：
Yu Hong [1 ]
Yu-Ping Liang [1 ]
Wei-Qi Chen [1 ]
Liu-Xia You [2 ]
Qing-Feng Ni [1 ]
Xiu-Yun Gao [1 ]
Xiao-Rong Lin [2 ]
机构：
[1] Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University
[2] Department of Clinical Laboratory, the Second Affiliated Hospital of Fujian Medical University
来源：
International Journal of Ophthalmology | 2021年 / 14卷 / 05期
关键词：
retinitis pigmentosa; heme oxygenase-1; ARPE-19; cell; mechanism;
D O I：
暂无
中图分类号：
R774.1 [视网膜疾病];
学科分类号：
100212 ;
摘要：
AIM: To investigate the protective effect of heme oxygenase-1(HO-1) against HO-induced apoptosis in human ARPE-19 cells. METHODS: The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by HO. Functional experiments including cell counting kit-8(CCK-8) assay, flow cytometry(FCM) and mitochondrial membrane potential assay were conducted. RESULTS: The ultrastructure of ARPE-19 cells was observed using transmission electron microscope(TEM). It was found that exogenous HO-1 significantly ameliorated HO-induced loss of cell viability, apoptosis and intracellular levels of reactive oxygen species(ROS) in ARPE-19 cells. The overexpression of HO-1 facilitated the transfer of nuclear factor erythroid-2-related factor 2(Nrf2) from cytoplasm to nucleus, which in turn upregualted expressions HO-1 and B-cell lymphoma-2(Bcl-2). Furthermore, HO-1 upregulation further inhibited HO-induced release of cysteinyl aspartate specific proteinase-3(caspase-3). CONCLUSION: Exogenous HO-1 protect ARPE-19 cells against HO-induced oxidative stress by regulating the expressions of Nrf2, HO-1, Bcl-2, and caspase-3.
引用
收藏
页码：649 / 655
页数：7
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