Expression of differential nitric oxide synthase isoforms in human gastric mucosa infected with Helicobacter pylori

被引：0
|
作者：
屠振兴
龚燕芳
丁华
许国铭
李兆申
满晓华
机构：
[1] Changhai Hospital
[2] Second Military Medical University
[3] Shanghai 200433
[4] China
[5] Department of Gastroenterology
来源：
Journal of Medical Colleges of PLA | 2003年 / 05期
基金：
中国国家自然科学基金;
关键词：
H; pylori; nitric oxide synthase; immunohistochemistry;
D O I：
暂无
中图分类号：
R573 [胃疾病];
学科分类号：
1002 ; 100201 ;
摘要：
Objective: To study the relationship between nitric oxide synthase ( NOS) expression in human gastric mu-cosa and Helicobacter pylori (H. pylori) infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H. pylori infection was confirmed by Giems staining and bacteria culture under mi-croaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: (1) The positive rate of H. pylori infection was 66. 7% (22/33) . (2) iNOS positive staining in inflammatory cells was detected in 77. 3% (17/22) of samples with H. pylori and 27. 3% (3/11) without H. pylori infection (P < 0.01). (3) eNOS expression in inflammatory cells was found in 77. 3% (17/22) of samples with H. pylori and 18.2% (2/11) without H. pylori infection ( P < 0. 01) . (4) Nitrotyrosine expression in inflammatory cells was observed in 59. 1% ( 13/22) of samples with H. pylori and 54.5%(6/11) without H. pylori infection (P>0.05). (5) Moderate a
引用
收藏
页码：286 / 289
页数：4
相关论文
共 50 条
  • [1] Differential Expression of Human Beta Defensin 2 and 3 in Gastric Mucosa of Helicobacter pylori-Infected Individuals
    Bauer, Bianca
    Wex, Thomas
    Kuester, Doerthe
    Meyer, Thomas
    Malfertheiner, Peter
    HELICOBACTER, 2013, 18 (01) : 6 - 12
  • [2] Localization of constitutive isoforms of nitric oxide synthase in the gastric glandular mucosa of the rat
    Price, KJ
    Hanson, PJ
    Whittle, BJR
    CELL AND TISSUE RESEARCH, 1996, 285 (01) : 157 - 163
  • [3] Increased expression of inducible nitric oxide synthase and peroxynitrite in Helicobacter pylori gastric ulcer
    Sakaguchi, AA
    Miura, S
    Takeuchi, T
    Hokari, R
    Mizumori, M
    Yoshida, H
    Higuchi, H
    Mori, M
    Kimura, H
    Suzuki, H
    Ishii, H
    FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (7-8) : 781 - 789
  • [4] Endothelial nitric oxide synthase (eNOS) is not upregulated in gastric mucosa of Helicobacter pylori (H. pylori)-positive patients with type 2 diabetes mellitus
    Lazaraki, G.
    Kountouras, J.
    Metallidis, S.
    Vrettou, E.
    Alevizos, M.
    Tzioufa, V.
    Dokas, S.
    Germanidis, G.
    Zavos, C.
    Nikolaidis, P.
    DIGESTIVE AND LIVER DISEASE, 2009, 41 (04) : 253 - 262
  • [5] Lipopolysaccharide of Helicobacter pylori protects gastric mucosa via generation of nitric oxide
    Brzozowski, T
    Konturek, PC
    Sliwowski, Z
    Drozdowicz, D
    Pajdo, R
    Stachura, J
    Hahn, EG
    Konturek, SJ
    JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1997, 48 (04): : 699 - 717
  • [6] p53 expression in the gastric mucosa of patients infected with Helicobacter pylori
    Morales-Fuentes, G. A.
    Zarate-Osorno, A.
    Quinonez-Urrego, E. E.
    Antonio-Manrique, M.
    Martinez-Garcia, C. L.
    Figueroa-Barojas, P.
    Zamorano-Orozco, Y.
    Leal-Osuna, S. E.
    Martinez-Camacho, C.
    Mejia-Cuan, L. A.
    Rivera-Nava, C. A.
    Sanchez-Chavez, X.
    Ramirez-Ramirez, M. A.
    REVISTA DE GASTROENTEROLOGIA DE MEXICO, 2013, 78 (01): : 12 - 20
  • [7] Effect of Helicobacter pylori infection on gastric mucosal pathologic change and level of nitric oxide and nitric oxide synthase
    Wang, Yong-Fu
    Guo, Chun-Lin
    Zhao, Li-Zhen
    Yang, Guo-An
    Chen, Peng
    Wang, Hong-Kun
    WORLD JOURNAL OF GASTROENTEROLOGY, 2005, 11 (32) : 5029 - 5031
  • [8] Differential activity of nitric oxide synthase in human nasal mucosa and polyps
    Ramis, I
    Lorente, J
    RoselloCatafau, J
    Quesada, P
    Gelpi, E
    EUROPEAN RESPIRATORY JOURNAL, 1996, 9 (02) : 202 - 206
  • [9] Expression of nitric oxide synthase isoforms in human pregnant myometrium at term
    Bao, SZ
    Rai, J
    Schreiber, J
    JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION, 2002, 9 (06) : 351 - 356
  • [10] Expression of nitric oxide synthase isoforms in the human placenta is not altered by labor
    Kakui, K
    Sagawa, N
    Itoh, H
    Yura, S
    Korita, D
    Takemura, M
    Nuamah, MA
    Fujii, S
    ENDOCRINE JOURNAL, 2003, 50 (05) : 535 - 544
12345