THE REMOVAL OF A POTENT INHIBITOR OF Taq POLYMERASE

被引：0
|
作者：
刘敬忠
机构：
[1] PRC
[2] Beijing 100730
[3] Institute of Basic Medical Sciences
[4] Chinese Academy of Medical Sciences
来源：
Chinese Science Bulletin | 1991年 / 01期
关键词：
PCR (polymerase chain reaction); Taq DNA polymerase; inhibitor of Taq polymerase; purification of genomic DNA; Sephadex G50;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
PCR is a rapid method for amplifying a defined sequence from small amounts of genomic DNA or mRNA. There is a potent inhibitor of Taq polymerase in some samples of genomic DNA that blocks amplifying but is nevertheless a good substrate for restriction enzymes. The inhibition cannot be overcome by extracting with phenol and chloroform and
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页码：66 / 68
页数：3
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