Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I

被引：0

作者：

LIAO MINGXIANG LIU DONGYUAN ZUO JIN AND FANG FUDENational Laboratory of Medical Molecular Biology Institute of Basic MedicalSciences ^{[2
]}

Institute of laboratory Animal Science Chinese Academy of MedicalSciences Peking Union Medical College Beijing

机构：

来源：

BiomedicalandEnvironmentalSciences | 2002年 / 01期

关键词：

Glutathione S-transferase P enhancer I; Yeast one-hybrid system trans-actionfactor;

D O I：

暂无

中图分类号：

Q501 [生物的化学成分和结构];

学科分类号：

071010 ; 081704 ;

摘要：

Objective To detect the trans-factors specifically binding to the strong enhancerelement (GPEI) in the upstream of rat glutathione S-transferase P (GST-P) gene. MethodsYeast one-hybrid system was used to screen rat lung MATCHMAKER cDNA library toidentify potential trans-factors that can interact with core sequence of GPEI(cGPEI).Electrophoresis mobility shift assay (EMSA) was used to analyze the binding of trans-factors to cGPEI. Results cDNA fragments coding for the C-terminal part of thetranscription factor c-Jun and rat adenine nucleotide translocator (ANT) were isolated. Thebinding of c-Jun and ANT to GPEI core sequence were confirmed. Conclusions Rat c-juntranscriptional factor and ANT may interact with cGPEI. They could play an important rolein the induced expression of GST-P gene.

引用

页码：36 / 40

页数：5