In [3], a kind of matrix-valued rational interpolants (MRIs) in the form of R;(x)=M(x)/D(x) with the divisibility condition D(x)|‖M(x)‖;, was defined, and the characterization theorem and uniqueness theorem for MRIs were proved. However this divisibility condition is found not necessary in some cases. In this paper, we remove this restricted condition, define the generalized matrix-valued rational interpolants (GMRIs) and establish the characterization theorem and uniqueness theorem for GMRIs. One can see that the characterization theorem and uniqueness theorem for MRIs are the special cases of those for GMRIs. Moreover, by defining a kind of inner product, we succeed in unifying the Samelson inverses for a vector and a matrix.