Alternative splicing of VEGFA,APP and NUMB genes in colorectal cancer

被引：6
作者：
Yi-Jun Zhao [1 ]
Hua-Zhong Han [1 ]
Yong Liang [2 ]
Chen-Zhang Shi [1 ]
Qing-Chao Zhu [1 ]
Jun Yang [1 ]
机构：
[1] Department of Surgery,Shanghai Jiao Tong University,Affiliated Sixth People’s Hospital
[2] Department of Gastrointestinal Surgery,the Affiliated Hospital of Xuzhou Medical College
来源：
World Journal of Gastroenterology | 2015年 / 21期
关键词：
Alternative splicing; Amyloid beta precursor protein; Colorectal cancer; Numb homolog; Vascular endothelial growth factor A;
D O I：
暂无
中图分类号：
R735.35 [];
学科分类号：
100214 ;
摘要：
AIM:To investigate alternative splicing in vascular endothelial growth factor A(VEGFA),amyloid beta precursor protein(APP),and Numb homolog(NUMB) in colorectal cancer(CRC).METHODS:Real-time quantitative reverse transcriptase polymerase chain reaction(q RT-PCR) and PCRrestriction fragment length polymorphism analyses were performed to detect the expression of VEGFA,APP,and NUMB mR NA in 20 CRC tissues and matched adjacent normal tissues,as well as their alternative splicing variants.RESULTS:q RT-PCR analysis revealed that the expression of APP,NUMB,and VEGFA 165 b m RNA were significantly downregulated,while VEGFA m RNA was upregulated,in CRC tissues(all P < 0.05).PCRrestriction fragment length polymorphism analysis revealed that the expression of VEGFA 165a/b in CRC tissues was significantly higher than in adjacent normal tissues(P < 0.05).Compared with adjacent normal tissues,the expression of NUMB-PRRS in CRC tissues was significantly decreased(P < 0.05),and the expression of NUMB-PRRL was increased(P < 0.05).CONCLUSION:Alternative splicing of VEGFA,APP,and NUMB may regulate the development of CRC,and represent new targets for its diagnosis,prognosis,and treatment.
引用
收藏
页码：6550 / 6560
页数：11
相关论文
共 14 条
[1]  
Colorectal cancer statistics, 2014[J] . Rebecca Siegel,Carol DeSantis,Ahmedin Jemal.CA A Cancer Journal for Clinicians . 2014 (2)
[2]  
Protecting the hedgerow: p53 and Hedgehog pathway interactions[J] . Louisa Ho,Benjamin Alman.Cell Cycle . 2010 (3)
[3]   RNA and Disease [J].
Cooper, Thomas A. ;
Wan, Lili ;
Dreyfuss, Gideon .
CELL, 2009, 136 (04) :777-793
[4]  
Expression analysis of VEGF-A and VEGF-B: Relationship with clinicopathologicalparameters in bladder cancer[J] . Sylvie Fauconnet,Stephane Bernardini,Isabelle Lascombe,Guillaume Boiteux,Anne Clairotte,Franck Monnien,Eric Chabannes,Hugues Bittard.Oncology Reports . 2009 (6)
[5]   Alternative splicing variant of vascular endothelial growth factor-A is a critical prognostic factor in non-small cell lung cancer [J].
Matsuyama, Masahiro ;
Chijiwa, Tsuyosht ;
Inoue, Yoshimasa ;
Abe, Yoshiyuki ;
Nishi, Masatake ;
Miyazaki, Noriyuki ;
Furukawa, Datsuke ;
Mukai, Masaya ;
Suemizu, Hiroshi ;
Sekido, Yasutomo ;
Ueyama, Yoshito ;
Nakamura, Masato .
ONCOLOGY REPORTS, 2009, 22 (06) :1407-1413
[6]   Recombinant human VEGF165b protein is an effective anti-cancer agent in mice [J].
Rennel, Emma S. ;
Hamdollah-Zadeh, Maryam A. ;
Wheatley, Edward R. ;
Magnussen, Anette ;
Schueler, Yvonne ;
Kelly, Sara P. ;
Finucane, Ciara ;
Ellison, David ;
Cebe-Suarez, Stephanie ;
Ballmer-Hofer, Kurt ;
Mather, Stephen ;
Stewart, Lorna ;
Bates, David O. ;
Harper, Steven J. .
EUROPEAN JOURNAL OF CANCER, 2008, 44 (13) :1883-1894
[7]   Alternative splicing and disease [J].
Kim, Eddo ;
Goren, Amir ;
Ast, Gil .
RNA BIOLOGY, 2008, 5 (01) :17-19
[8]   A switch in numb Isoforms is a critical step in cortical development [J].
Bani-Yaghoub, Mahmud ;
Kubu, Chris J. ;
Cowling, Rebecca ;
Rochira, Jennifer ;
Nikopoulos, George N. ;
Bellum, Stephen ;
Verdi, Joseph M. .
DEVELOPMENTAL DYNAMICS, 2007, 236 (03) :696-705
[9]   Diabetic retinopathy is associated with a switch in splicing from anti- to pro-angiogenic isoforms of vascular endothelial growth factor [J].
Perrin, RM ;
Konopatskaya, O ;
Qiu, Y ;
Harper, S ;
Bates, DO ;
Churchill, AJ .
DIABETOLOGIA, 2005, 48 (11) :2422-2427
[10]   The effect of diet on risk of cancer [J].
Key, TJ ;
Allen, NE ;
Spencer, EA ;
Travis, RC .
LANCET, 2002, 360 (9336) :861-868
12