Construction of Large Human Single-chain Antibody Phage Display Library

被引：0

作者：

张志超

机构：

来源：

High Technology Letters | 2002年 / 03期

关键词：

phage display; single chain antibody; diversity;

D O I：

暂无

中图分类号：

R-5 [];

学科分类号：

10 ;

摘要：

A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.

引用

页码：1 / 4

页数：4

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