THE ACTIVATION OF PROTO-ONCOGENE C-HA-RAS ANDTHE CONTROL OF ITS EXPRESSION IN HUMANSTOMACH CANCER

被引：0
作者：
邓国仁
Wu
武纯静
苗晶
李华
徐宁志
金晓明
鄂征
机构：
来源：
ChineseJournalofCancerResearch | 1988年 / 00期
关键词：
THE ACTIVATION OF PROTO-ONCOGENE C-HA-RAS ANDTHE CONTROL OF ITS EXPRESSION IN HUMANSTOMACH CANCER; DNA;
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摘要：
Activated oncogene c-Ha-ras in stomach cancer tissues was identified by DNA transfection. The point mutations were determined in five DNA samples (from three solid tumors of patients with gastro-carcinoma and two cell lines) out of twelve (eight solid tumors and four cell lines) by the restriction fragment length polymorphysm (RFLP) assay. The expressions of c-Ha-ras were enhanced to higher levels (10-20 times) in two cell lines. The lengths of variable tandem repetition (VTR) sequences downstream from the c-Ha-ras coding region were compared for different DNA samples. Their ability to enhance c-Ha-ras expression and their transforming ability are discussed.
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页码：5 / 8
页数：4
相关论文
共 5 条
  • [1] Shih C,at al.Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell . 1982
  • [2] Blair DG,et al.Tumorigenesis by transfected cell in nude mice: A new method for detecting cellular transforming gene. Progress in Clinical and Biological Research . 1983
  • [3] Rappaport E,et al.Growth inhibition of human tumor cells in soft agar cultures by treatment with low levels of adenosine 5’-triphosphate. Cancer Research . 1983
  • [4] Deng G,et al.Activated c-Ha-ras oncogene with a guanine to thymine transversion at the twelfth codon in human stomach cancer cell lines. Cancer Research . 1987
  • [5] Southern EM.Detection of specific sequence among DNA fragments separated by electrophoresis. Journal of Molecular Biology . 1975
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