Crystal structure determination of basic phospholipase Afrom venom of Agkistrodon halys pallas by molecular replacement method

被引：0

作者：

孟五一

林政炯

周元聪

机构：

[1] State Key Laboratory of Biomacromolecules

[2] Institute of Biophysics

[3] Chinese Academy of Sciences

[4] Beijing

[5] China

[6] Shanghai Institute of Biochemistry

[7] Shanghai

来源：

Science in China(Series C:Life Sciences) | 1996年 / 06期

关键词：

phospholipase A2 from Agkistrodon halys pallos; hemolysis; molecular replacement method; crystal structure;

D O I：

暂无

中图分类号：

Q45 [内分泌生理学];

学科分类号：

071003 ;

摘要：

<正> Basic phospholipase A2 (BPLA2) from the venom of Agkistrodon halys pallas has a strong ability to hemolyze erythrocytes. The asymmetrical unit of P212121 crystal of BPLA2 contains two molecules. Self-rotation function was used to study the orientation relationship of these two molecules. Cross-rotation and translation functions were then used to determine the orientations and positions of the two molecules in the unit cell. The model building and preliminary structure refinement were carried out. The result shows that the two molecules in the asymmetrical unit of orthorhombic crystal are related by a non-crystallographic 2-fold symmetry axis.

引用

页码：584 / 591

页数：8