Structure and sequence analysis of influenza A virus nucleoprotein

被引:0
作者
NG Andy Ka-Leung
SHAW Pang-Chui
机构
[1] MolecularBiotechnologyProgramme,DepartmentofBiochemistryandCentreforProteinScienceandCrystallography,TheChi-neseUniversityofHongKong
关键词
Influenza H5N1; nucleoprotein; oligomerization; RNA binding;
D O I
暂无
中图分类号
R373 [人体病毒学(致病病毒)];
学科分类号
100103 ; 100705 ;
摘要
Influenza A virus nucleoprotein (NP) forms homo-oligomers and multiple copies of NP wrap around genomic RNA, along with a trimeric polymerase making up ribonucleoprotein (RNP) complex. Sequence comparison of more than 2500 influenza A NP showed that this protein contains 30.1 % of polymorphic residues. NP is composed of a head and a body domain and a tail loop/ linker region. The head domain is more conserved than the body domain, as revealed from the structure-based sequence alignment. NP oligomerization is mediated by the insertion of the non-polymorphic and structurally conserved tail loop of one NP molecule to a groove of another NP. The different form of NP oligomers is due to the flexibility of the polymorphic linkers that join the tail loop to the rest of the protein. The RNA binding property of NP is known to involve the protruding element and the flexible basic loop between the head and body domains, both having high degree of primary sequence conservation. To bind RNA, NP may first capture the RNA by the flexible basic loop and then the RNA is clamped by the protruding element.
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页码:439 / 449
页数:11
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