Purification and Characterization of a New Ribosome Inactivating Protein from Cinchonaglycoside C-treated Tobacco Leaves

被引：0

作者：

Yanmei LiYantao JiaZhongkai ZhangXiaoying ChenHongping HeRongxiang Fang and Xiaojiang Hao State Key Laboratory of Photochemistry and Plant Resources in West ChinaKunming Institute of Botanythe Chinese Academy of SciencesKunming China ^{[1
,4
,2
,3
,2
,1
,2
,1
,1
,650204
]}

State Key Laboratory of Plant GenomicsInstitute of Microbiologythe Chinese Academy of SciencesBeijing China ^{[2
,100080
]}

Laboratory of Plant VirologyYunnan Academy of Agricultural SciencesKunming China ^{[3
,650223
]}

The Graduate School of the Chinese Academy of SciencesBeijing China ^{[4
,100049
]}

机构：

来源：

Journal of Integrative Plant Biology | 2007年 / 09期

关键词：

antiviral protein; Cinchonaglycoside C(1); coat protein; medical plant; ribosome inactivating protein; salicylic acid; tobacco mosaic virus;

D O I：

暂无

中图分类号：

S435.72 [烟草病虫害];

学科分类号：

090401 ; 090402 ;

摘要：

<正> A new ribosome-inactivating protein(RIP)with a molecular weight of 31 kDa induced by Cinchonaglycoside C(1)designatedCLP31,was isolated from tobacco leaves.Analysis of this protein sequence indicated that it belongs to the RIP family and itwas distinct from the other plant RIPs reported previously at its N-terminal amino acid sequence.CIP31 can directly impairsynthesis of coat protein(CP)of tobacco mosaic virus(TMV),which resulted in inhibition of TMV long distance movementand multiplication in tobacco plants at concentrations of ng/mL.Furthermore,no toxicity was shown to the growth andfertility of the plants.CIP31 was synthesized only in the presence of Cinchonaglycoside C(1)and was independent of thesalicylic acid(SA)signal pathway.We provided evidence for the SA-independent biological induction of resistance.

引用

页码：1327 / 1333

页数：7

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