hCAP-18/LL-37基因转染对RAW264.7细胞活化功能的影响

被引：0

作者：

刘超 ^{[1
,2
]}

袁红艳 ^{[1
]}

焦淑凡 ^{[1
]}

余磊 ^{[1
]}

常雅萍 ^{[1
]}

机构：

[1] 吉林大学白求恩医学院免疫学系

[2] 山东省淄博市疾病预防控制中心

来源：

中国生物制品学杂志 | 2011年 / 24卷 / 02期

关键词：

抗菌肽; hCAP-18/LL-37; 转染; 巨噬细胞; 活化; 脂多糖类;

D O I：

10.13200/j.cjb.2011.02.35.liuch.004

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

目的探讨抗菌肽hCAP-18/LL-37基因转染对巨噬细胞RAW264.7活化功能的影响。方法将重组质粒pcD-NA4/Myc-His-hCAP-18/LL-37瞬时转染RAW264.7细胞,MTT法检测细胞的增殖活性;中性红吞噬试验检测细胞的吞噬能力;RT-PCR法分析细胞活化相关分子CD80、CD86、TLR4及细胞因子IL-1β、TNF-αmRNA的转录。结果 hCAP-18/LL-37基因转染可促进经脂多糖(Lipopolysaccharide,LPS)处理的RAW264.7细胞的增殖活性与吞噬能力(P<0.05);可上调RAW264.7细胞CD80、CD86、TLR4、IL-1β、TNF-α基因mRNA的转录水平。结论 hCAP-18/LL-37基因转染可通过促进RAW264.7细胞增殖活性、吞噬能力及活化相关分子表达,调控巨噬细胞的活化功能。

引用

页码：153 / 156

页数：4

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