Capillary electrophoresis with electrochemiluminescence detection for the analysis of quinolone drugs and pharmacokinetics study

A novel method for the determination of two quinolone drugs norfloxacin（NOR）and levofloxacin（LVX）was described by capillary electrophoresis with electrochemiluminescence detection.The good relationship（r≥0.9991）between peak area and concentration of analytes was established over two orders of magnitude.The limits of detection（LOD,S/N=3）in standard solution are 4.8×10;mol/L for NOR and 6.4×10;mol/L for LVX,respectively.The limits of quantitation（LOQ,S/N=10）in real human urine samples are 1.2×10;mol/L for NOR and 1.4×10;mol/L for LVX,respectively.The present method was successfully applied to the determination of NOR and LVX in human urine and the study of pharmacokinetics of NOR.