Molecular detection of Xanthomonas oryzae pv.oryzae, Xanthomonas oryzae pv. oryzicola, and Burkholderia glumae in infected rice seeds and leaves

被引：6

作者：

Wen Lu ^{[1
]}

Luqi Pan ^{[1
]}

Haijun Zhao ^{[2
]}

Yulin Jia ^{[3
]}

Yanli Wang ^{[4
]}

Xiaoping Yu ^{[1
]}

Xueyan Wang ^{[1
]}

机构：

[1] Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Science, China Jiliang University

[2] Institute of Nuclear-Agricultural Science, Zhejiang University

[3] United States Department of Agriculture, Agricultural Research Service, Dale Bumpers National Rice Research Center (USDA-ARS DB NRRC)

[4] Institute of Plant Protection and Microbe, Zhejiang Academy of Agricultural Sciences

来源：

The Crop Journal | 2014年 / 2卷 / 06期

关键词：

Xanthomonas oryzae pv.oryzae; X.oryzae pv.oryzicola; B.glumae; Pathogen detection; PCR;

D O I：

暂无

中图分类号：

S435.111.4 [侵（传）染性病害];

学科分类号：

090401 ; 090402 ;

摘要：

The polymerase chain reaction(PCR) is particularly useful for plant pathogen detection. In the present study, multiplex PCR and SYBR Green real-time PCR were developed to facilitate the simultaneous detection of three important rice pathogens, Xanthomonas oryzae pv.oryzae, X. oryzae pv. oryzicola, and Burkholderia glumae. The unique PCR primer sets were designed from portions of a putative glycosyltransferase gene of X. oryzae pv. oryzae, an Avr Rxo gene of X. oryzae pv. oryzicola, and an internal transcribed spacer(ITS) sequence of B. glumae. Using a multiplex PCR assay, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected in one PCR reaction that contained the newly developed primer set mix. Using SYBR Green real-time PCR assays, X. oryzae pv. oryzae, X. oryzae pv. oryzicola, and B. glumae were detected at 1, 1, and 10 fg μL-1, respectively. These newly designed molecular assays are sensitive and could be reliable tools for pathogen detection and disease forecasting.

引用

页码：398 / 406

页数：9

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