Experimental Study on Gufusheng in Treatment of Steroid-Induced Ischemic Necrosis of Femoral Head in Rabbits

Objective:To explore the mechanisim of compound Gufusheng in treatment of steroid-induced ischemicnecrosis of femoral head(SINFH).Thirty-two rabbits were randomly divided into a blank control group(n=12)and a model group(n=20).The rabbits of the model group were established by intramuscularinjection of prednisone acetate(0.32mg/kg/d)for 8 weeks,and 2 rabbits in each group were killed at the6th and the 8th week respectively to investigate whether the model was successfully prepared.Thenremainder in the model group was again randomly divided into group A(Gufusheng group,n=8)andgroup B(model group,n=8),while the rest of rabbits in the blank group constituted group C(n=8).Therabbits in group A were given Gufusheng Decoction(骨复生煎液)by intragastric perfusion,and therabbits in group B and C were fed with normal saline.After treatment for 4 weeks,the seruminsulin-like growth factor-1(IGF-1)and tumor necrosis factor-α(TNF-α)levels in the rabbits of the 3groups were determined by radioimmunoassay(RIA).The serum TNF-α decreased significantly and theIGF-1 level increased significantly in the Gufusheng group as compared with the model group(bothP<0.01).Compound Gufusheng can prevent SINFH and promote repair of the tissue of necrosis offemoral head possibly via decreasing the TNF-α and increasing IGF-1 level.