Visual Detection of Murray Valley Encephalitis Virus by Reverse Transcription Loop-Mediated Isothermal Amplification

被引：0

作者：

GONG Rui ^{[1
,2
]}

WANG Han Hua ^{[1
]}

QIN Hong ^{[1
,2
]}

GUO Xiao Ping ^{[1
,2
]}

MA Xue Jun ^{[3
]}

机构：

[1] Hubei International Travel Healthcare Center

[2] National Key Lab of Ports Infectious Disease Detection,Hubei Entry-Exit Inspection and Quarantine Bureau of China

[3] Key Laboratory for Medical Virology,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention

来源：

BiomedicalandEnvironmentalSciences | 2015年 / 28卷 / 03期

关键词：

RT; LAMP; Visual Detection of Murray Valley Encephalitis Virus by Reverse Transcription Loop-Mediated Isothermal Amplification; RNA; gene; Bank;

D O I：

暂无

中图分类号：

R512.3 [脑炎及脑脊髓膜炎];

学科分类号：

100401 ;

摘要：

A sensitive reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay was developed for rapid visual detection of Murray valley encephalitis virus(MVEV)infection.The reaction was performed in one step in a single tube at 63°C for 60 min with the addition of the hydroxynaphthol blue(HNB)dye prior to amplification.The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold

引用

页码：227 / 230

页数：4

共 7 条

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