Isolation and identification of compounds from Phaleria macrocarpa(Scheff.) Boerl fruit extract

Objective: To identify and isolate the chemical compounds of Phaleria macrocarpa（P.macrocarpa） fruit ethanolic extract.Methods: Dried fruit of P.macrocarpa was extracted with 90% ethanol and partitioned between n-hexane/H;O and ethyl acetate/H;O.The organic layer was fractionated by various stationary phase and identified by using combined data of 1D [(proton nuclear magnetic resonance（NMR）, carbon-13 NMR)], 2D-NMR（heteronuclear multiplequantum correlation and heteronuclear multiple-bond correlation）, and mass spectrum.Results: Purification of n-hexane and ethyl acetate fractions from ethanolic extract of P.macrocarpa fruit resulted in isolation of nine compounds.Conclusions: A new compound was isolated and identified as glyceryl pentacosanoate.Also, two xanthones, which are 1,7-dihydroxy-3,6-dimethoxyxanthone and 1,6,7-trihydroxy-3-methoxyxanthone, are firstly reported to be isolated from P.macrocarpa.