Immune Regulation of Interleukin-27 in Malignant Pleural Effusion

被引:0
作者
Li Shi
You Wen-Jie
Zhang Jian-Chu
Zhou Qiong
Shi Huan-Zhong
机构
[1] Tongji Medical College
[2] China
[3] Hubei 430022
[4] Department of Respiratory and Critical Care Medicine
[5] Wuhan
[6] Huazhong University of Science and Technology
[7] Union Hospital
[8] Key Laboratory of Pulmonary Diseases of Health Ministry
关键词
Interleukin-27; Lung Cancer; Malignant Pleural Effusion; Signal Transduction;
D O I
暂无
中图分类号
R561.3 [水胸];
学科分类号
1002 ; 100201 ;
摘要
Background: Interleukin (IL)-27 has been reported to have anti-proliferate and anti-angiogenic activities on cancer cells. However, the involvement of IL-27 in malignant pleural effusion (MPE) remains unknown. Thus, in this research, we compared the immune functions of IL-27, interferon (IFN)-γ, and IL-17 on lung cancer cells and revealed the regulatory mechanism of IL-27 in MPE.Methods: The distribution of IL-27 in both MPE and blood was evaluated by enzyme-linked immunosorbent assay and flow cytometry. The expressions of cytokine receptors and the levels of the phosphorylated signal transducer and activator of transcription (STAT) signalings were detected by flow cytometry. As well as the effects of proliferation, apoptosis, migration, and adherent activity of IL-27, IFN-γ, and IL-17 on lung cancer cells were also explored.Results: The expression of IL-27 was increased in MPE when compared with blood (147.3 ± 25.1 pg/ml vs. 100.3 ± 13.9 pg/ml,P = 0.04). IL-27 was noted to suppress both proliferation (18.33 ± 0.21 vs. 27.77 ± 0.88,P = 0.0005) and migration (1.82 ± 0.44 vs. 3.13 ± 0.07,P = 0.04) of A549 cells, but obviously promoted apoptosis of A549 cells (9.47 ± 1.14 vs. 4.96 ± 0.17,P = 0.02) by activating STAT1 signaling. Interestingly, IL-27 played totally opposite effects on A549 cells by activating STAT3 pathway. Moreover, IL-27 exerted different intercellular adherent activities of A549 cells to pleural mesothelial cell monolayer by activating different STAT signalings.Conclusions: IL-27 might exert an important immune regulation on lung cancer cells in human pleural malignant environment.
引用
收藏
页码:1932 / 1941
页数:10
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