Determinations of Five Kinds of Secondary Metabolites from Different Parts of Piper sarmentosum Roxb.

[Objectives] To establish the determination methods for the contents of total flavonoids,total alkaloids,total saponins,total phenolics and total tannins from different parts of P.sarmentosum.[Methods] The content of total flavonoids was measured by the NaNO;-Al（NO;）;-NaOH colorimetry; the content of total alkaloids was measured by the bromocresol green acid dyes colorimetric assay; the content of total spaonins was measured by the vanillin- acetic acid acid dyes colorimetric assay; the content of total phenolics was measured by the FolinCiocalteu assay; the content of total tannins was measured by the phospho molybdenum tungstic acid- casein colorimetry.[Results]The average recovery rate of rutin was 98.11% and RSD was 0. 59%,the contents of total flavonoids from roots,stems and leaves of P.sarmentosum were 0. 25%,0.33% and 1.18% respectively; the average recovery rate of berberine hydrochloride was 99.62% and RSD was 1.52%,the contents of total alkaloids from roots,stems and leaves of P.sarmentosum were 0. 01%,0.03% and 0.19% respectively; the average recovery rate of ginsenoside Re was 99.21% and RSD was 1.06 %,the contents of total saponins in roots,stems and leaves of P.sarmentosum were 4.13%,1.71% and 4.33% respectively; the average recovery of gallic acid was 97.95% and RSD was 0.88 %,the contents of total phenol in roots,stems and leaves of P.sarmentosum were 0.68%,1.08% and 2.42% respectively; the average recovery of gallic acid was 97.92% and RSD was 3.14%,the contents of total tannins in roots,stems and leaves of P.sarmentosum were 0.72%,1.21% and 1.69% respectively.[Conclusions] These five proposed methods are precise,accurate and reproducible,and they are suitable for the determinations of total flavonoids,total alkaloids,total saponins,total phenolics and total tannins in P.sarmentosum.