Disposable amperometric biosensors based on xanthine oxidase immobilized in the Prussian blue modified screen-printed three-electrode system

被引：0

作者：

TENG YuanJieCHEN ChenZHOU ChangXiangZHAO HongLi LAN MinBo Shanghai key Laboratory of Functional Materials Chemistry

Research Center of Analysis TestEast China University of Science and TechnologyShanghai China ^{[200237
]}

机构：

来源：

Science China(Chemistry) | 2010年 / 12期

关键词：

xanthine oxidase; xanthine; hypoxanthine; screen-printed three-electrode system; Prussian blue;

D O I：

暂无

中图分类号：

TP212.3 [生物传感器、医学传感器];

学科分类号：

080202 ;

摘要：

The screen-printed three-electrode system was applied to fabricate a new type of disposable amperometric xanthine oxidase biosensor.Carbon-working,carbon-counter and Ag/AgCl reference electrodes were all manually printed on the polyethylene terephthalate substrate forming the screen-printed three-electrode system by the conventional screen-printing process.As a mediator,Prussian blue could not only catalyze the electrochemical reduction of hydrogen peroxide produced from the enzyme reaction,but also keep the favorable potential around 0 V.The optimum operational conditions,including pH,potential and temperature,were investigated.The sensitivities of xanthine and hypoxanthine detections were 13.83 mA/M and 25.56 mA/M,respectively.A linear relationship was obtained in the concentration range between 0.10μM and 4.98μM for xanthine and between 0.50μM and 3.98μM for hypoxanthine.The small Michaelis-menten constant value of the xanthine oxidase biosensor was calculated to be 3.90 μM.The results indicate that the fabricated xanthine oxidase biosensor is effective and sensitive for the detection of xanthine and hypoxanthine.

引用

页码：2581 / 2586

页数：6

共 16 条

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