Fermentation Kinetics of Media Optimization for the Production of Alpha Amylase by a New Isolate of Aspergillus Oryzae

被引：0

作者：

IkramulHaq ^{[1
]}

Roheena Abdullah ^{[1
]}

Hamid Mukhtar ^{[1
]}

Muhammad Nauman Aftab ^{[2
]}

机构：

[1] Institute of Industrial Biotechnology, Government College University

[2] Bioinformatics Laboratory, Institute of Biophysics, Chinese Academy of Sciences

来源：

微生物学通报 | 2007年 / 04期

关键词：

Alpha amylase; Kinetics; Aspergillus oryzae; Fermentation media;

D O I：

10.13344/j.microbiol.china.2007.04.030

中图分类号：

TQ920.1 [基础理论];

学科分类号：

081703 ; 082203 ;

摘要：

The present study is concerned with the isolation and screening of different strains of Aspergillus oryzae for the production of alpha amylase. Ninety strains were isolated from soil and tested for the production of alpha amylase in shake flasks. Of all the strains tested, Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35 gave maximum production of alpha amylase. Different culture media were screened for the production of alpha amylase by these two strains. M1 medium containing starch, yeast extract, NH4Cl, MgSO4·7H2O and CaCl2 gave the maximum production of alpha amylase by both the strains Aspergillus oryzae GCB-32 and Aspergillus oryzae GCB-35.Kinetic analysis revealed that the values of product yield coefficient（Y_ p/x ） and specific product yield coefficient（qp） were found highly significant（p≤0.05） when medium M1 was used for the enzyme production.

引用

页码：740 / 744

页数：5