Expression Profiling of MicroRNAs in Hippocampus of Rats Following Traumatic Brain Injury

被引：0

作者：

孙婷怡 ^{[1
]}

陈晓瑞 ^{[1
]}

刘子龙 ^{[1
]}

赵丽丽 ^{[1
]}

江永祥 ^{[2
]}

屈国强 ^{[1
]}

王荣帅 ^{[1
]}

黄锶哲 ^{[1
]}

刘良 ^{[1
]}

机构：

[1] Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology

[2] Forensic Expertise Centre of Xiling District Public Security Bureau

来源：

JournalofHuazhongUniversityofScienceandTechnology(MedicalSciences) | 2014年 / 34卷 / 04期

关键词：

forensic clinical medicine; traumatic brain injury; microRNA; microarray technique; hippocampus;

D O I：

暂无

中图分类号：

D919 [法医学];

学科分类号：

0838 ; 1001 ; 1012 ;

摘要：

The changes of microRNA expression in rat hippocampus after traumatic brain injury(TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.

引用

页码：548 / 553

页数：6

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TRANSCRIPTION-AUSTIN, 2013, 4 (04) :177-191

[2]

miR-221/222 Compensates for Skp2-Mediated p27 Degradation and Is a Primary Target of Cell Cycle Regulation by Prostacyclin and cAMP .2 Castagnino Paola,Kothapalli Devashish,Hawthorne Elizabeth A,Liu Shu-Lin,Xu Tina,Rao Shilpa,Yung Yuval,Assoian Richard K. PloS one . 2013

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