Interaction of zinc finger ZNF191(243—368) and its I323W and R327W mutants with oligonucleotides by florescence spectrum

被引：0
|
作者：
周晶
刘玉奇
汤李明
滕昕辰
唐宁
黄仲贤
机构：
[1] School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China ,Department of Chemistry, Fudan University, Shanghai 200433, China ,Department of Chemistry, Fudan University, Shanghai 200433, China ,Department of Chemistry, Fudan Un
[2] Department of Chemistry, Fudan University, Shanghai 200433, China
来源：
Science in China(Series B) | 2003年 / 01期
基金：
中国国家自然科学基金;
关键词：
zinc finger protein ZNF191(243—368); site-directed mutagenesis; fluorescence spectrum; protein-DNA inter-action;
D O I：
暂无
中图分类号：
Q51 [蛋白质];
学科分类号：
摘要：
Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.
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页码：64 / 74
页数：11
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