Expression pattern of GASA,downstream genes of DELLA,in Arabidopsis

Separation and functional research of related components involved in gibberellins(GAs) signaling are important to clarify the mechanism of GA functioning.Research on the downstream components of DELLA,the key factor of the GA signaling pathway,is limited at present.GASA(GA-Stimulated in Arabidopsis) family contains 15 genes usually regulated by GA in Arabidopsis thaliana.All GASA proteins have a cleavable signal peptide in N terminus and a conserved GASA domain including 12 cysteines in C terminus.RT-PCR analysis revealed that the expression of GASA4 and GASA6 were down-regulated,but GASA1 and GASA9 were up-regulated in the DELLA mutants,gai-t6 and rga-24,as well as the double mutant,consisting with the results that GASA4 and GASA6 were induced,but GASA1 and GASA9 were inhibited by exogenous GA3.In addition,the expression patterns of other GASA genes were regulated by GA and ABA,separately or cooperatively.Most of GASA genes were expressed in roots,stems,leaves,flowers and developing siliques.GUS gene driven by the promoters of GASA6,GASA7,GASA8,GASA9,GASA10,GASA11 and GASA12 were used as reporters and it was found that all GASA genes expressed in the growing and differentiating organs and abscission zones,suggesting the role of these genes in cell growth and differentiation.This study provided an important basis for functional study of the GASA gene family in the GA and ABA signaling pathway.