Study with microarrays of the differential gene expression profiles of glioblastoma

被引：0

作者：

杨志林

徐如祥

姜晓丹

柯以铨

罗成义

金莹

胡庚熙

机构：

来源：

Journal of Medical Colleges of PLA | 2001年 / 04期

关键词：

glioma; microarray; geneexpression;

D O I：

暂无

中图分类号：

R730.264 [神经组织瘤];

学科分类号：

100214 ;

摘要：

Objective:Thisstudyaimsto screenthedifferentiallyexpressedgenesof glioblastomausingmicroarraytech-nique.Methods:Specimensof glioblastomaandnormalbraintissuewereobtainedfrompathologicallyconfirmedpatients.A cDNAmicroarraycomprising14000clonescoveringthewholesetsof theretro-transcriptionalproductsof themRNAs of variousgliomasandthoseof normalbraintissueswas established,withwhichthedifferencesin geneexpressionbe-tweenglioblastomaandnormalbraintissueswereinvestigated.Results:Itwas foundthat94genesweremorethan3-fold differentiallyexpressedwith298morethandoubledintheglioblastomaincomparisonwiththenormalbraintissue.Some over-expressedgenesintheglioblastomawerescarcelyexpressedinnormalbraintissues,andseveralnovelgenesthatmay havebiologicalrelevanceintheprocessof gliomagenesiswereidentified.Conclusion:Microarraytechniquecombined withrelevantcDNArepositorycanfacilitaterapidlarge-scaleidentificationof potentialtargetgenesfordiagnosisandther-apy of glioma.

引用

页码：269 / 273 +277

页数：6